![]() The dot blot technique’s main principle is based on the hybridization method, where a specific radioactive probe will bind with the desired DNA, RNA or protein.It’s official – biologists DO have a sense of humor, well some of them at least. Therefore, a dot blot technique is a method of detecting DNA, RNA and protein from the different sample characterized by the different spots. The specific sequence of a gene can also be detected in a transgenic individual.Dot blot technique is widely used to detect protein concentration.It does not provide a basis for comparing an original and a modified target biomolecule within the same slot.Dot blot method does not give any qualitative information about the target biomolecules’ size and molecular weight. ![]() Dot blot technique aids in direct blotting of biomolecule onto the membrane.It does not involve immobilization of the biomolecules from a gel matrix to the filter membrane. ![]()
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